Here we going to compare both test, purpose ,principle and procedure to make  difference between two test of coagulation profile  test (ex PT, APTT)  which are used   for screening extrinsic clothing factor and For screening intrinsic clothing factor for monitor the treatment

Difference between PT and APTT

Name of test	Prothrombin time	Activated partial Prothrombin time
Factors	extrinsic clothing	intrinsic clothing
Names	X, V, II, I, and XII	I, II, IX, X, XI, and XII.

Difference between PT and APTT

Difference between prothombin time ( PT) and  activated partial prothombin time  (APTT)

here we  going to compare following points

• Purpose
• principle
• factors
• requirement
• regent
• procedure
• normal velue
• prolonged value

Difference between PT and APTT
Full form	Prothrombin time			Activated partial Prothrombin time
Also know as	PT TEST			APTT
Purpose	For screening extrinsic clothing system			For screening  intrinsic clothing system
Name of factors	clotting factors X, V, II, I, and XII			Factors I, II, IX, X, XI, and XII.
Principle	Plasma is added with thromboplastin and calcium chloride regent  @ 37 degree and time taken to clot formation known as Prothrombin time			Kaolin (surface activator) and platelet substrate (phospholipids) are incubated with citrate plasma at 37 degree for time specified in the method. Cacl2 is add in the test tube &the time taken for the mixture to clot is note.
Requirement	Water bath or incubator, stopwatch,			Water bath or incubator, stopwatch,
Regent	Thromboplastin calcium chloride regent (combined regent)			Kaolin /platelet substrate mixture, calcium chloride.
Specimen	Citrate plasma (Tri-sodium citrate anticoagulant)			Citrate plasma (Tri-sodium citrate anticoagulant)
procedure	Thromboplastin calcium chloride regent (combined regent)		0.250 ML or 250ul	Keolin /platelet substitute		0.2 ml or 200ul
				plasma		0.1ml or 100  ul
	Citrate plasma		0.05ml or 50 ul	Mix and incubate for  exactly 2 minutes
				Tilting the tubes at interval
	Hold the tube in water bath and start the stop watch and note down			Calcium chloride	0.1ml or 100  ul
				Hold the tube in water bath and start the stop watch and note down  the time
result	the time taken for clot formation then convert the clotting  time in INR using table given by manufacture and reported			Calculate the average patent result and calculate
Reference range	In second	2-16 second		Normal range
	INR	0.8-1-2		30-40 sec
	INR (worfarin treatment)	2-3		35-50 sec
Prolonger INR	Treatment with oral anticouglent blood such as warfirin			Prolonger APTT
				DIC
	Liver disease			Deficiency of clothing factor
	DIC			hemophilia
	Hemolytic disease of new born
	Rarely deficiency of these factor

Question could be put in exams regrinding this topic are are given below

write short notes on

• Prothombin time
• Activated partial Prothrombin time
• write about coagulation function test

MCQ could be given from this topics are

• normal value of PT  test
• normal value of APTT test
• what is INR
• regents are use in PT or APTT test
• anticoagulant used in this test

if you know the answer please comment  below