Manual Cell Counting Using Neubauer Chamber | RBC Count | WBC Count
Manual Cell Counting Using Neubauer Chamber
Hematology lab here we are using fur blood cells count
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Microscope
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RBC pipette
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WBC pipette
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Improved Neubauer,s, s counting chamber for RBC count and WBC
count
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| RBC pipette used for RBC and platelet count |
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| WBC pipette used for WBC and sperm count |
Difference between WBC pipette and RBC pipette |
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WBC pipette |
RBC pipette |
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Marking |
0.5-1-11 |
0.5-1-101 |
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Bead |
White color |
Red color |
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Bulb |
Small bulb |
Large bulb |
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Used
for |
WBC count, semen analysis |
RBC, Platelet count |
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Microscope here we are using 3 types of microscope
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Simple microscope
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Monocular compound light microscope
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Binocular compound light microscope
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| Microscope for blood cells count Simple microscope, Monocular, Binocular compound light microscope |
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Difference |
Simple microscope |
compound light microscope |
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lens |
Only Convex lens |
Two convex lenses, objective and eyepiece, are used |
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objectives |
The object is placed within the focal length of the convex lens |
The objective is placed
beyond the focal length of the objective lens |
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Magnifications |
Less than a compound microscope |
Much greater than a simple microscope |
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used |
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Now we are going to see the pipette the parts of the pipette having a
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Steam
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Bulb rubber tube and
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Mouthpiece
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There is one plastic material which is present inside the bulb
is known as bead
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Here are the marking for example 0.5-1 and 101 for RBC
pipette
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WBC pipette marking 0.5 and 11
Q.1 what is the use of the bulb?
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Bulb is present for mixing blood and diluting fluid
Q.2 what is the use of beads?
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beat helps in mixing the blood with diluting fluid indicates the
bulb whether it is dry or wet helps to identify whether the pipette is an RBC pipette or WBC pipette
Q.3 how to identify RBC pipette
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RBC pipette markings of the RBC pipette is 0.5 -1 and 101
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Large bulb as compared to WBC pipette
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Red-bead so these all are the identification points of the RBC
pipette
Q.4 how to identify WBC pipette
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How we are going to identify the WBC pipette
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Markings 0.5-1 and 11 small bulbs is compared to an RBC pipette
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White bread and white mouthpiece
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These all are the identification points of the WBC pipette
Q.5 how to identify the improved Neubauer,s counting chamber
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These are counting areas there are two counting areas are present
in the improved Neubauer,s, s counting chamber
Counting area in neubauer, s chamber
- improved neubauer counting chamber
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| improved neubauer counting chambe |
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Counting area of improved Neubauer counting chamber table is 3 by 3 mm square it is divided by two
vertical and two horizontal lines into 9 squares here are four squares are for
WBC count and one center square is for RBC count
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| counting area in improved Neubauer counting chamber for RBC Count |
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Every WBC count square is 1 by 1 mm which again divided into 16
small squares by three vertical and three horizontal lines every small square
is 1 by 4 mm in length and width
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| Counting area in improved Neubauer counting chamber for WBC Count |
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RBC square have triple borders the central RBC square is divided
into 25 square which having triple borders its beak RBC square is 1 by 5 mm in
length and width
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Its big RBC square is again divided into 16 small squares by three
vertical and three horizontal lines and all the small squares having a single
borderline
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| Counting area in improved Neubauer counting chamber for RBC Count |
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it’s one by 20 mm in length and width when we are going to put a
coverslip over the improved new bars counting chamber the distance between the
counting area and the coverslip is around 1 by 10 mm this one by 10mm is
known as depth
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| Put coverslip over-improved Neubauer counting chamber |
Let’s solve some MCQ questions
that will help to evaluate yourself that how much you have learned yet.